Research

As experts in the development of new diagnostic assessments, our research scientists continually explore new technologies for use in the assessment of health status, health risk and age-related diseases.

Innovation

Our goal is to develop new and better assays and methods of analysis. We investigate new ways of presenting patient-friendly test results within a unique, proprietary graphic presentation of Kronos optimal ranges that are relevant, understandable and backed by scientific evidence.

Service

We provide unprecedented attention to the needs of every doctor, scientist and patient, and are available for consultations to ensure laboratory results are meaningful for each patient.

Excellence

Utilizing the support and guidance of distinguished physicians and world-renowned scientists, we continue to set new industry standards for the practice of Optimal Health Medicine.

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We Are The Trusted Experts We Keep Things Simple

Kronos is dedicated to the improvement of human health and longevity. Through our analytical laboratory, patient clinic and external collaborations, Kronos focuses on the development and application of innovative science to evaluate health and prevent age-related diseases. Kronos is a full-service, quality-driven clinical reference and contract research organization specializing in custom assay development, test validation, diagnostic testing services and the development of novel diagnostic tests. Specialty laboratory services are provided to pharmaceutical companies, biotechnology companies and other organizations engaging in clinical trials and basic research. The laboratory’s areas of expertise are Cardiovascular Disease, Alzheimer’s disease, Inflammation, Oxidative Stress, Endocrinology and Nutrition. Services provided include custom assay development, esoteric and traditional testing services, custom data retrieval and reporting, multi-center trial support, investigator support, advanced specimen storage, tracking and retrieval, custom sample collection and shipment kit development.

The Kronos Laboratory facility houses a complete clinical reference and research laboratory with state-of-the-art automated equipment, analytical equipment and manual testing areas as well as sample storage areas providing both short and long-term sample storage capability. An advanced Laboratory Information Management (LIM) system allows for customized reporting to meet the requirements of individual study protocols.

In addition to collaborating on and supporting the research being conducted by colleagues and clients, Kronos conducts its own basic and translational research in the fields of optimal health and human aging. Specific areas of interest include oxidative stress, hormone therapy, cancer screening, and age related diseases such as Alzheimer’s disease. One ongoing project focuses exclusively on human aging and directed by Kronos Laboratory is the Kronos Longitudinal Aging Study or KLAS.

Completed Projects

Completed Kronos Science research includes, but is not limited to the following projects

Development of High Throughput Testing of Highly Unsaturated Fatty Acids (HUFA)

The goal of this project is to develop a high throughput method for testing of highly unsaturated fatty acids (HUFAs) in red blood cell (RBC) membranes. This assay will focus on the omega 3 fatty acids EPA and DHA as a percent of total HUFA, which has been shown to be an excellent predictor of cardiovascular disease risk.

Development of an Oxidative Stress Assay Panel

The goal of this study is to create a panel of oxidative stress assays measuring DNA, RNA, lipid and protein oxidation. Markers on this panel now include 8-hydroxy-d’-deoxyguanosine (8OHdG), 8-hydroxy-guanosine (8oxoGuo), and 5-hydroxymethyl-2-deoxyuridine (5OHmU), markers of oxidative damage to DNA & RNA, 8-Iso-Prostaglandiin-F(2alpha-IV), 8-Iso-Prostaglandiin-F(2alpha-VI) and 2,3-Dinor-8-Iso-Prostaglandin-F(2alpha), markers of lipid peroxidation, and dityrosine and nitro-tyrosine, markers of protein oxidation. This project has been extremely successful and is the basis of ongoing research on oxidative stress.

Development of an Ionization Radiation Oral Protector

This project was conducted in collaboration with the University of Windsor. The goal of the project was to measure the free radical damage created by radiation exposure during routine clinical examinations using whole body CT scanning. The damage was found to be measurable, but the method of measurement was too variable to be useful as a screening tool. A more stable, yet equally sensitive method of measuring minute levels of DNA damage is being pursued.

The Primate Longevity Determinant Gene Comparative Project

The Primate LDG project was to search for key genes that could account for human’s comparatively long lifespan. The question asked was what is unique about human biology that can account for an unusually long lifespan compared to all other mammalian species and in particular, the great apes. This study involved comparison of both biochemical markers and genome-wide gene expression profiling to examine differences between the two species. Results are currently being documented for publication. This project was performed in collaboration with the Primate Foundation of Arizona, Southwest Foundation for Biomedical Research (SFBR) and the Yerkes National Primate Research Center.

DNA Damage Markers in Smokers, Ex-Smokers and Non-Smokers; A Comparison Study

The objective of this study was to determine if the Kronos Science DNA damage assay panel (8OHdG, 8oxoGuo, and 5OHmU) along with an isoprostane (PGF2α) could provide a reliable assessment of ongoing rates of free radical damage in lipid membranes. A distinction between levels of damage in smokers, ex-smokers, and non-smokers was used to clinically validate these assays.

Impact of Environmental and Physiological Stress on Cytokines and Lymphocyte Subclasses in U.S. Marines

In collaboration with the Naval Health Research Center, this study attempted to identify patterns of cytokines and lymphocyte subclasses in United States Marines exposed to prolonged periods (up to six weeks) of extreme environmental and physiological stress. The goal was to find patterns that were predictive of susceptibility to illness and infection. Follow-up studies in collaboration with the NHRC are under discussion.